Yes, if you click the "Let Us Assign Your GTIN" button, your GS1 US account will incorporate your GS1 Company Prefix, reserve the right number of digits for the Item Reference, and generate the check digit.
If you already know the Item Reference you want to enter for this GTIN, click the "Choose a Specific GTIN" button. Enter the Item Reference, then click the "Get GTIN Check Digit" button, and the check digit is calculated for you.
If you already know the GS1 Company Prefix AND the Item Reference but want to learn how to calculate the check digit manually (outside of your GS1 US account), visit the GS1 US Check Digit Calculator page to calculate the check digit.